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Recombinant LSD1 / KDM1A protein
添加时间:2019-11-15

  Recombinant LSD1 / KDM1A protein

  原价:¥3,304.60

  现价:¥2,379.31

  品牌:Active Motif

  货号:31426

  销量:126

  配送:由沃鎏波洱发货,并提供售后服务

  规格:20 μg

Recombinant LSD1 / KDM1A protein

  沃鎏波洱(www.valubio.com)致力于打造亚太地区最大的、生命医学科研领域的、集进口科研试剂、耗材、仪器设备销售,品牌推广、技术服务等为一体的一站式电子商务平台,为生命医学提供专业、高效、优质服务。

  沃鎏波洱平台主营生命医学及生物科技相关的产品销售、技术服务等业务,现有600+个品牌,580万+种商品;产品覆盖免疫学、细胞生物学、分子生物学、蛋白质组学等诸多热点学科领域。沃鎏波洱平台将资源和服务进行有效整合,具备了强大的市场获取能力,凭借独特的业务模式获得资本市场青睐。

  概述货号31426

  使用方法Recombinant LSD1 / KDM1A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

  Specific Activity: H3K4me2 and H3K4me1 demethylase.

  Catalytic Ability: > 600 turnovers / enzyme molecule.

  Histone Demethylase Assay Conditions: 3.3 μM H3K4me2 (1-21aa) peptide was incubated with 5 nM KDM1A / LSD1 in reaction buffer containing 50 mM TrisCl, pH 8.0, 0.02% Triton X-100, 50 nM FAD for 1 hours at room temperature. MALDI-TOF were used for detection.

  性能供应商Active Motif

  背景LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.

  存放说明Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

  外观Full length recombinant KDM1A / LSD1 protein was expressed in Sf9 cells at a concentration of 0.4 μg/μl in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP.

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