Recombinant PCAF (715-829) protein
使用方法Recombinant PCAF (715-829) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
背景P300/CBP-associated factor (PCAF), also known as K (lysine) acetyltransferase 2B (KAT2B), is a transcriptional coactivator associated with p53 that contains acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. PCAF competes with E1A for binding to p300/CBP, which associate with various sequence-specific factors involved in cell growth and/or differentiation, including c-Jun and the adenoviral oncoprotein E1A. PCAF exerts histone acetyltransferase activity in association with core histones (H3 and H4) and with nucleosome core particles, and functions to promote transcriptional activation. PCAF has also been shown to inhibit cell cycle progression and to counteract the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, PCAF is recruited by the viral protein TAT to regulate TAT’s transactivating activity and may help induce chromatin remodeling of proviral genes.
存放说明Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
外观Recombinant PCAF (715-829) protein expressed in E. coli at a concentration of 3 mg/ml in 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.