Recombinant HDAC4 protein
使用方法Recombinant HDAC4 is suitable for use in histone deacetylase (HDAC) assays. A good starting point is 2 to 5 ng of enzyme per assay.
背景HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs)involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.
In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11).
Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.
存放说明Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
外观100 µg of recombinant HDAC4 protein expressed in E. coli at a concentration of 2.86 mg/ml in 25mM HEPES pH7.5, 200mM KCl, 1mM DTT and 50% glycerol.