Recombinant GCN5 (726-837) protein
使用方法Recombinant GCN5 (726-837) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
背景GCN5, also known as K (lysine) acetyltransferase 2A (KAT2A), is a component of the ATAC complex, which is a complex that specifically exerts histone acetyltransferase (HAT) activity on histones H3 and H4. GCN5 acts as a histone acetyltransferase to promote transcriptional activation. In the case of HIV-1 infection, it is recruited by the viral protein TAT and regulates TAT's transactivating activity. In addition, evidence suggests GCN5 may play a role in the induction of chromatin remodeling of proviral genes. GCN5 also functions as a repressor of NFκB by promoting ubiquitination of the NFκB subunit RelA in a HAT-independent manner. GCN5 contains a bromodomain that recognizes acetylated histone lysine residues and functions as a ‘reader’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
存放说明Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
外观100 µg of recombinant GCN5 (726-837) protein expressed in E. coli at a concentration of 4 mg/ml in 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.