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Recombinant JARID1A / KDM5A protein
添加时间:2019-11-14

  Recombinant JARID1A / KDM5A protein

  原价:¥3,304.60

  现价:¥2,379.31

  品牌:Active Motif

  货号:31431

  销量:186

  配送:由沃鎏波洱发货,并提供售后服务

  规格:20 μg

Recombinant JARID1A / KDM5A protein

  沃鎏波洱(www.valubio.com)致力于打造亚太地区最大的、生命医学科研领域的、集进口科研试剂、耗材、仪器设备销售,品牌推广、技术服务等为一体的一站式电子商务平台,为生命医学提供专业、高效、优质服务。

  沃鎏波洱平台主营生命医学及生物科技相关的产品销售、技术服务等业务,现有600+个品牌,580万+种商品;产品覆盖免疫学、细胞生物学、分子生物学、蛋白质组学等诸多热点学科领域。沃鎏波洱平台将资源和服务进行有效整合,具备了强大的市场获取能力,凭借独特的业务模式获得资本市场青睐。

  概述货号31431

  使用方法Recombinant JARID1A / KDM5A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

  Specific Activity: H3K4me3 demethylase.

  Catalytic rate: > 60 turnovers/ enzyme molecule

  Histone Demethylase Assay Conditions: 3.3 μM H3K4me3 (aa 1-21) peptide was incubated with different concentrations of recombinant JARID1A / KDM5A protein in reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP for 1 hours at room temperature. HTRF and MALDI-TOF were used for detection.

  性能供应商Active Motif

  背景Lysine (K)-specific demethylase 5A (KDM5A), also known as Jumonji, AT rich interactive domain 1A (JARID1A), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5A demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5A may function to stimulate transcription mediated by nuclear receptors and may be involved in transcriptional regulation of HOX proteins during cell differentiation. Also, KDM5A may be involved in the transcriptional repression of cytokines such as CXCL12. It has been shown to bind directly with the Retinoblastoma (pRb) protein which regulates cell proliferation. KDM5A also interacts with Rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis.

  存放说明Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

  外观Full length recombinant JARID1A / KDM5A protein expressed in Sf9 at a concentration of 0.5 mg/ml in 40 mM HEPES pH 7.5, 300 mM NaCl and 5% glycerol.