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Recombinant Tet1 protein
添加时间:2019-11-14

  Recombinant Tet1 protein

  原价:¥6,277.50

  现价:¥4,519.80

  品牌:Active Motif

  货号:31363

  销量:186

  配送:由沃鎏波洱发货,并提供售后服务

  规格:25 ug

Recombinant Tet1 protein

  沃鎏波洱(www.valubio.com)致力于打造亚太地区最大的、生命医学科研领域的、集进口科研试剂、耗材、仪器设备销售,品牌推广、技术服务等为一体的一站式电子商务平台,为生命医学提供专业、高效、优质服务。

  沃鎏波洱平台主营生命医学及生物科技相关的产品销售、技术服务等业务,现有600+个品牌,580万+种商品;产品覆盖免疫学、细胞生物学、分子生物学、蛋白质组学等诸多热点学科领域。沃鎏波洱平台将资源和服务进行有效整合,具备了强大的市场获取能力,凭借独特的业务模式获得资本市场青睐。

  概述货号31363

  使用方法Recombinant Tet1 protein is suitable for use in cytosine oxygenase assays. A good starting point is 0.5 to 5 μg of enzyme per assay incubated with 0.5 μg of substrate in 50 mM HEPES, pH 8, 50 μM Fe(NH4)2(SO4)2, 2 mM ascorbate, and 1 mM alpha-ketoglutarate for 3 hr at 37°C.

  NOTE:Reaction buffers should made fresh.

  性能供应商Active Motif

  背景Ten-eleven Translocation Gene Protein 1 (Tet1) is a DNA-binding protein that modulates DNA methylation and gene transcription via hydroxylation of 5-methylcytosine (5-mC). Tet1 is a cytosine oxygenase that catalyzes the conversion of the modified genomic base 5-mC into 5-hydroxymethylcytosine (5-hmC). Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. Tet1 is responsible for initiating the enzymatic deamination process leading to cytosine demethylation. Tet1 preferentially binds to CpG-rich sequences at the promoters of both transcriptionally active and Polycomb-repressed genes and modulates gene silencing induced by cytosine methylation by controlling the levels of 5-mC and 5-hmC at gene promoters. Tet1 may have the dual function of repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Tet1 is important for stem cell maintenance and specification and is involved in the balance between pluripotency and lineage commitment.

  存放说明Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

  外观25 µg recombinant Tet1 protein expressed in E. coli supplied in a buffer of 20 mM Tris 8.0, 300 mM NaCl, 2 mM DTT and 5% glycerol.