Recombinant MLL4-SET protein
使用方法Recombinant MLL4-SET is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
背景Myeloid/lymphoid or mixed-lineage leukemia 4 (MLL4) is a Trithorax-group protein that function collectively to promote gene expression. MLL4 is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL4 plays a central role in β-globin locus transcription regulation by being recruited by Nfe2. MLL4 is also required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that precedes resumption of meiosis, oocyte survival and normal zygotic genome activation. MLL4 may also be involved in human cancer as its expression has been shown to be upregulated in solid tumor cell lines.
存放说明Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
外观20 µg of recombinant MLL4-SET protein expressed in E. coli at a concentration of 0.4 mg/ml in 20 mM Tris-HCl pH 7.4, 150 mM NaCl and 20% glycerol.