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Recombinant JARID1C / KDM5C protein
添加时间:2019-11-14

  Recombinant JARID1C / KDM5C protein

  原价:¥3,304.60

  现价:¥2,379.31

  品牌:Active Motif

  货号:31433

  销量:199

  配送:由沃鎏波洱发货,并提供售后服务

  规格:20 μg

Recombinant JARID1C / KDM5C protein

  沃鎏波洱(www.valubio.com)致力于打造亚太地区最大的、生命医学科研领域的、集进口科研试剂、耗材、仪器设备销售,品牌推广、技术服务等为一体的一站式电子商务平台,为生命医学提供专业、高效、优质服务。

  沃鎏波洱平台主营生命医学及生物科技相关的产品销售、技术服务等业务,现有600+个品牌,580万+种商品;产品覆盖免疫学、细胞生物学、分子生物学、蛋白质组学等诸多热点学科领域。沃鎏波洱平台将资源和服务进行有效整合,具备了强大的市场获取能力,凭借独特的业务模式获得资本市场青睐。

  概述货号31433

  使用方法Recombinant JARID1C / KDM5C is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

  Specific Activity: H3K4me3 demethylase.

  Histone Demethylase Assay Conditions:

  50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP, 100 nM Recombinant JARID1C / KDM5C protein, and 3.3 μM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

  性能供应商Active Motif

  背景Lysine (K)-specific demethylase 5C (KDM5C), also known as Jumonji, AT rich interactive domain 1C (JARID1C), is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. It is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5C participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and CoREST pAb at neuron-restrictive silencer elements. Mutations in KDM5C have been associated with X-linked mental retardation.

  存放说明Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

  外观Full length recombinant JARID1C / KDM5C protein expressed in Sf9 at a concentration of 0.85 mg/ml in 40 mM HEPES pH 7.4, 150 mM NaCl and 20% glycerol.