【Cell期刊同款商品推荐】之Cell封面故事 | 一个与内质网交织的无膜细胞器促进3’UTR介导的蛋白质间相互作用
 

膜细胞器和无膜细胞器在细胞内形成区室以实现对胞内生化反应的时空控制。在本期Cell杂志中，Weirui Ma（马为锐博士）和Christine Mayr描述了一种新的基于RNA的无膜细胞器，即TIS颗粒，它与内
质网交织在一起，形成一个亚细胞区室—TIGER结构域—具有特定的生物物理和生物化学环境。该区室促进特异性3’UTR介导的蛋白质间相互作用。
论文链接：https://www.cell.com/cell/fulltext/S0092-8674(18)31315-1
 
大约一半的人类基因会产生具有3’UTR（3’非翻译区）的mRNA。通过介导蛋白质-蛋白质相互作用，3’UTR可实现具有相同氨基酸序列的蛋白质的多功能性。
 
在研究蛋白质特征的信息如何从3’UTR转移到蛋白质时，我们发现广泛表达的RNA结合蛋白TIS11B形成了无膜细胞器，我们称为TIS颗粒（TIS granule），其富含编码膜蛋白的mRNA，这些mRNA包含有多个富含AU的元素。
 
TIS颗粒形成与内质网（ER）交织的网状结构，该关联产生亚细胞区室—TIGER结构域—具有与细胞质不同的生物理化环境。该区室促进了3’UTR介导的SET与膜蛋白的相互作用，进而增加蛋白质的膜表达和功能多样性，包括CD47和PD-L1等蛋白。
 
TIGER结构域使无法在外部建立的特异性的和与功能相关的蛋白质-蛋白质相互作用能够在亚细胞区室形成。


Cell同款
 

	商品名称	品牌	货号	规格	图片	商品ID
抗体	Anti-GFP antibody	abcam	ab13970	100ul	/images/brand//1531903327532.jpeg	4582555
	Monoclonal Anti-alpha-Tubulin antibody produced in mouse clone DM1A, ascites fluid	Sigma-Aldrich	T9026-100UL	100ul	/images/brand//1532685366227.png	2518805
	Monoclonal Anti-Actin antibody produced in mouse clone AC-40, ascites fluid	Sigma-Aldrich	A4700-100UL	EA	/images/brand//1532685366227.png	2518812
	Anti-HuR Antibody	Merck Millipore	07-1735	100ul	/images/merckmillipore_images/MM_NF-07-1735.jpg	7884823
	HuR抗体 (3A2)	Santa Cruz	sc-5261	200ug/ml	/images/brand//1532685336540.jpg	2212640
	Anti-KHSRP antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody	Sigma-Aldrich	SAB4200566-200UL	200ul	/images/brand//1532685366227.png	2531138
	Anti-FXR1 antibody produced in rabbit Prestige Antibodies(R) Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol soluti	Sigma-Aldrich	HPA018246-100UL	100ul	/images/brand//1532685366227.png	2584954
	hnRNP A1 抗体 (D-4)	Santa Cruz	sc-374526	200ug/ml	/images/brand//1532685336540.jpg	2254975
	Anti-CD47 antibody [EPR4150(2)]	abcam	ab108415	100ul	/images/brand//1531903327532.jpeg	7047124
	Anti-SET antibody [EPR12973]	abcam	ab181990	100ul	/images/brand//1531903327532.jpeg	7046986
	ZFP36L1 Antibody	proteintech	12306-1-AP	50ul	/images/brand//1522646715462.jpg	6047299
	Anti-mCherry antibody [1C51]	abcam	ab125096	100ul	/images/brand//1531903327532.jpeg	4631081
	Monoclonal Anti-FUS antibody produced in mouse clone FUS-4, tissue culture supernatant	Sigma-Aldrich	SAB4200478-200UL	200ul	/images/brand//1532685366227.png	61467
	Anti-FUBP3 (center) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution	Sigma-Aldrich	SAB1300583-100UG	100ug	/images/brand//1532685366227.png	2527404
	PerCP-Cy5.5 mouse anti-CD47	BD PharMingen	561261	50Tst		2059829
	Alexa Fluor 647 mouse anti-CD47	BD PharMingen	561249	50Tst		2059828
	IRDye® 800CW Donkey anti-Rabbit IgG (H + L), 0.5 mg	LI-COR	926-32213	0.5mg	/images/brand//1528189294723.png	7888383
	Goat anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 633	Invitrogen	A-21103	500ul	/images/brand//1531905104649.jpg	2613473
	Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594	Invitrogen	A-11037	500ul	/images/goods/61261.jpg	2617956
	Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568	Invitrogen	A-11004	500ul	/images/goods/61056.jpg	2613784
其它试剂	GFP-Trap_A beads	Chromotek	gta-100	100 rxns (2.5ml)	/images/1544423467468.png	2495532
	Dynabeads® MyOne™ Streptavidin C1 beads	Invitrogen	65002	10ml	/images/goods/54440.jpg	2616151
	Lipofectamine™ 2000 Transfection Reagent	Invitrogen	11668019	1.5ml	/images/1544424402268.jpg	3002205
	LiCor Odyssey blocking buffer	LI-COR	927-40000	500ml		7888318
	Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)	Thermo Scientific	78439	10ml	/images/goods/68477.jpg	2712934
	SeeBlue® Plus2 Pre-Stained Standard	Invitrogen	LC5925	500ul	/images/goods/53678.jpg	2711631
	NuPAGE® MES SDS Running Buffer (for Bis-Tris Gels only) (20X) (500 ml)	Invitrogen	NP0002	500ml	/images/brand//1531905104649.jpg	2712422
	NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 10-well	Invitrogen	NP0321PK2	2 gels (1 box)	/images/goods/53382.jpg	2604036
	NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 12-well	Invitrogen	NP0322BOX	10 gels (1 box)	/images/goods/53383.jpg	2604037
	NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5 mm, 15-well	Invitrogen	NP0336BOX	10 gels (1 box)	/images/goods/53391.jpg	2604046
	NuPAGE® Transfer Buffer (20X) (1 L)	Invitrogen	NP0006-1	1L	/images/goods/69565.jpg	2712428
	Q5 超保真 DNA 聚合酶	NEB	M0491L	500 units	/images/brand//1532685646128.jpg	2991692
	T4 DNA 连接酶	NEB	M0202L	100,000 units	/images/brand//1532685646128.jpg	2991885