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cell同款之封面故事
添加时间:2019-08-27
【Cell期刊同款商品推荐】之Cell封面故事 | 一个与内质网交织的无膜细胞器促进3’UTR介导的蛋白质间相互作用
 

膜细胞器和无膜细胞器在细胞内形成区室以实现对胞内生化反应的时空控制。在本期Cell杂志中,Weirui Ma(马为锐博士)和Christine Mayr描述了一种新的基于RNA的无膜细胞器,即TIS颗粒,它与内
质网交织在一起,形成一个亚细胞区室—TIGER结构域—具有特定的生物物理和生物化学环境。该区室促进特异性3’UTR介导的蛋白质间相互作用。
论文链接:https://www.cell.com/cell/fulltext/S0092-8674(18)31315-1
 
大约一半的人类基因会产生具有3’UTR(3’非翻译区)的mRNA。通过介导蛋白质-蛋白质相互作用,3’UTR可实现具有相同氨基酸序列的蛋白质的多功能性。
 
在研究蛋白质特征的信息如何从3’UTR转移到蛋白质时,我们发现广泛表达的RNA结合蛋白TIS11B形成了无膜细胞器,我们称为TIS颗粒(TIS granule),其富含编码膜蛋白的mRNA,这些mRNA包含有多个富含AU的元素。
 
TIS颗粒形成与内质网(ER)交织的网状结构,该关联产生亚细胞区室—TIGER结构域—具有与细胞质不同的生物理化环境。该区室促进了3’UTR介导的SET与膜蛋白的相互作用,进而增加蛋白质的膜表达和功能多样性,包括CD47和PD-L1等蛋白。
 
TIGER结构域使无法在外部建立的特异性的和与功能相关的蛋白质-蛋白质相互作用能够在亚细胞区室形成。


Cell同款
 
  商品名称 品牌 货号 规格 图片 商品ID
抗体
 
Anti-GFP antibody abcam ab13970 100ul /images/brand//1531903327532.jpeg 4582555
Monoclonal Anti-alpha-Tubulin antibody produced in mouse clone DM1A, ascites fluid Sigma-Aldrich T9026-100UL 100ul /images/brand//1532685366227.png 2518805
Monoclonal Anti-Actin antibody produced in mouse clone AC-40, ascites fluid Sigma-Aldrich A4700-100UL EA /images/brand//1532685366227.png 2518812
Anti-HuR Antibody Merck Millipore 07-1735 100ul /images/merckmillipore_images/MM_NF-07-1735.jpg 7884823
HuR抗体 (3A2) Santa Cruz sc-5261 200ug/ml /images/brand//1532685336540.jpg 2212640
Anti-KHSRP antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody Sigma-Aldrich SAB4200566-200UL 200ul /images/brand//1532685366227.png 2531138
Anti-FXR1 antibody produced in rabbit Prestige Antibodies(R) Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol soluti Sigma-Aldrich HPA018246-100UL 100ul /images/brand//1532685366227.png 2584954
hnRNP A1 抗体 (D-4) Santa Cruz sc-374526 200ug/ml /images/brand//1532685336540.jpg 2254975
Anti-CD47 antibody [EPR4150(2)] abcam ab108415 100ul /images/brand//1531903327532.jpeg 7047124
Anti-SET antibody [EPR12973] abcam ab181990 100ul /images/brand//1531903327532.jpeg 7046986
ZFP36L1 Antibody proteintech 12306-1-AP 50ul /images/brand//1522646715462.jpg 6047299
Anti-mCherry antibody [1C51] abcam ab125096 100ul /images/brand//1531903327532.jpeg 4631081
Monoclonal Anti-FUS antibody produced in mouse clone FUS-4, tissue culture supernatant Sigma-Aldrich SAB4200478-200UL 200ul /images/brand//1532685366227.png 61467
Anti-FUBP3 (center) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution Sigma-Aldrich SAB1300583-100UG 100ug
 
/images/brand//1532685366227.png 2527404
PerCP-Cy5.5 mouse anti-CD47 BD PharMingen 561261 50Tst   2059829
Alexa Fluor 647 mouse anti-CD47 BD PharMingen 561249 50Tst   2059828
IRDye® 800CW Donkey anti-Rabbit IgG (H + L), 0.5 mg LI-COR 926-32213   0.5mg /images/brand//1528189294723.png 7888383
Goat anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Invitrogen A-21103 500ul /images/brand//1531905104649.jpg 2613473
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 Invitrogen A-11037 500ul /images/goods/61261.jpg 2617956
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen A-11004 500ul /images/goods/61056.jpg 2613784
其它试剂
 
GFP-Trap_A beads Chromotek gta-100 100 rxns (2.5ml) /images/1544423467468.png 2495532
Dynabeads® MyOne™ Streptavidin C1 beads Invitrogen 65002 10ml /images/goods/54440.jpg 2616151
Lipofectamine™ 2000 Transfection Reagent Invitrogen 11668019 1.5ml /images/1544424402268.jpg 3002205
LiCor Odyssey blocking buffer LI-COR 927-40000 500ml   7888318
Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X) Thermo Scientific 78439 10ml /images/goods/68477.jpg 2712934
SeeBlue® Plus2 Pre-Stained Standard Invitrogen LC5925 500ul /images/goods/53678.jpg 2711631
NuPAGE® MES SDS Running Buffer (for Bis-Tris Gels only) (20X) (500 ml) Invitrogen NP0002 500ml /images/brand//1531905104649.jpg 2712422
NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 10-well Invitrogen NP0321PK2 2 gels (1 box) /images/goods/53382.jpg 2604036
NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 12-well Invitrogen NP0322BOX 10 gels (1 box) /images/goods/53383.jpg 2604037
NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.5 mm, 15-well Invitrogen NP0336BOX 10 gels (1 box) /images/goods/53391.jpg 2604046
NuPAGE® Transfer Buffer (20X) (1 L) Invitrogen NP0006-1 1L /images/goods/69565.jpg 2712428
Q5 超保真 DNA 聚合酶 NEB M0491L 500 units /images/brand//1532685646128.jpg 2991692
T4 DNA 连接酶 NEB M0202L 100,000 units /images/brand//1532685646128.jpg 2991885